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Protocol Name
In situ PCR using leaf epidermal peels
DOI:10.17504/protocols.io.p85dry6 RRID Copied  
PDF Report How to cite
Diep Ganguly, Asmini Athman 2019. In situ PCR using leaf epidermal peels. protocols.io dx.doi.org/10.17504/protocols.io.p85dry6
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.p85dry6

Authors: Diep Ganguly, Asmini Athman

Group: Pogson Genomics Group

Summary: Method for visualizing cell-localized gene expression patterns.Adapted from: Athman, A., Tanz, S.K., Conn, V.M., Jordans, C., Mayo, G.M., Ng, W.W., Burton, R.A., Conn, S.J., and Gilliham, M. (2014). Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue. Plant Methods 10: 29.Also, for more information on epidermal peels: Chen, Z.-H., Eisenach, C., Xu, X.-Q., Hills, A., and Blatt, M.R. (2012). Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis. Plant Methods 8: 15.

Affiliations: The Australian National University, University of Adelaide

Version: 1

Publication Date: 2019

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Source: Protocols.io