Resource Summary Report  New Search Previous Search Results

URL: https://dx.doi.org/10.17504/protocols.io.p33dqqn

Authors: Nicholas Leigh, Garrett Dunlap, Kimberly Johnson, Rachelle Mariano, Rachel Oshiro, Alan Y. Wong, Donald M. Bryant, Bess Miller, Jessica L. Whited

Summary: This protocol provides details on tissue harvest and fixation, RNA probe generation, and RNA in situ hybridization for use in sectioned axolotl tissue. This protocol was modified from Brent et al, A somitic compartment of tendon progenitors, Cell 2003

Associated Publications: Leigh ND, Dunlap GS, Johnson K, Mariano R, Oshiro R, Wong AY, Bryant DM, Miller BM, Ratner A, Chen A, Ye WW, Haas BJ, Whited JL, Transcriptomic landscape of the blastema niche in regenerating adult axolotl limbs at single-cell resolution. Nature Communications doi: 10.1038/s41467-018-07604-0

Affiliations: Brigham and Women's Hospital/Harvard Medical School, Brigham and Women's Hospital/Harvard Medical School, Brigham and Women's Hospital/Harvard Medical School, Brigham and Women's Hospital/Harvard Medical School, Brigham and Women's Hospital/Harvard Medical School, Brigham and Women's Hospital/Harvard Medical School, Brigham and Women's Hospital/Harvard Medical School, Brigham and Women's Hospital/Harvard Medical School, Brigham and Women's Hospital/Harvard Medical School

External URL: https://doi.org/10.1038/s41467-018-07604-0

Version: 1

Publication Date: 2018

Expand All Collapse