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Protocol Name
DOI:10.17504/protocols.io.p2xdqfn RRID Copied  
PDF Report How to cite
Antonio Real-Hohn 2018. Viral Genome Release and Native Viral Particle Conversion Measured Through Temperature Ramping in Real Time PCR Machine. protocols.io dx.doi.org/10.17504/protocols.io.p2xdqfn
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.p2xdqfn

Authors: Antonio Real-Hohn

Summary: This protocol was developed to use common wet lab device (Real Time PCR machine) to detect viral genome release and native viral particle conversion. We used temperature ramping for inducing genome uncoating and fluorescent probes for RNA/DNA and/or proteins, to detect genome and/or capsid changes.

Affiliations: Medizinische Universität Wien

Version: 1

Publication Date: 2018

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Data and Source Information

Source: Protocols.io