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Marlon Stoeckius, Peter Smibert 2018. Cell Hashing. protocols.io https://dx.doi.org/10.17504/protocols.io.nfzdbp6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.nfzdbp6
Authors: Marlon Stoeckius, Peter Smibert
Group: Human Cell Atlas Method Development Community
Summary: This protocol is for performing Cell Hashing only. Sample multiplexing and super-loading on single cell RNA-sequencing platforms.Cell Hashing uses a series of oligo-tagged antibodies against ubiquitously expressed surface proteins with different barcodes to uniquely label cells from distinct samples, which can be subsequently pooled in one scRNA-seq run. By sequencing these tags alongside the cellular transcriptome, we can assign each cell to its sample of origin, and robustly identify doublets originating from multiple samples.
Affiliations: New York Genome Center Technology Innovation Lab, New York Genome Center Technology Innovation Lab
External URL: https://cite-seq.com/cell-hashing/
Version: 1
Publication Date: 2018
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