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URL: https://dx.doi.org/10.17504/protocols.io.mixc4fn

Authors: Paul Dény, Frédéric Le Gal, Ségolène Brichler, Athénais Gerber, Paul Dény

Summary: PreC-C Primers encompass described PreC-C mutations: mutation G1896A in the PreC region and mutations A1762T and G1764A in C-gene promotor. Primers were modified according to literature-based alignment, in order to optimize the amplification of all HBV genotypes. Sequence analysis at position 1762, 1764 et 1896 allows to determine sample wild type or mutant sequence (i.e. HBeAg expression or not, respectively). It is also possible to describe others rare punctual mutations. Furthermore, the sequence analysis also allows assessing HBV genotyping.

Associated Publications: Komas NP, Ghosh S, Abdou-Chekaraou M, Pradat P, Hawajri NA, Manirakiza A, Laghoe GL, Bekondi C, Brichler S, Ouavéné J, Sépou A, Yambiyo BM, Gody JC, Fikouma V, Gerber A, Samarakoon NA, Alfaiate D, Scholtès C, Martel N, Gal FL, Pinto HL, Amri I, Hantz O, Durantel D, Lesbordes J, Gordien E, Merle P, Drugan T, Trépo C, Zoulim F, Cortay J, Kay AC, Dény P (2018) Hepatitis B and hepatitis D virus infections in the Central African Republic, twenty-five years after a fulminant hepatitis outbreak, indicate continuing spread in asymptomatic young adults. PLoS Negl Trop Dis 12(4): e0006377. doi: 10.1371/journal.pntd.0006377

Affiliations: , , , , Université Paris 13, Centre de Recherches en Cancérilogie de Lyon

External URL: https://doi.org/10.1371/journal.pntd.0006377

Version: 2

Publication Date: 2018

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