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Protocol Name
DOI:10.17504/protocols.io.kwscxee RRID Copied  
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Daniel Richter, Parinaz Fozouni, Michael Eisen, Nicole King 2017. Transcriptome sequencing of 19 diverse species of choanoflagellates. protocols.io dx.doi.org/10.17504/protocols.io.kwscxee
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.kwscxee

Authors: Daniel Richter, Parinaz Fozouni, Michael Eisen, Nicole King

Group: Ecology of Marine Plankton (ECOMAP) team - Roscoff, King Lab, Protist Research to Optimize Tools in Genetics (PROT-G)

Summary: The origin of animals, which occurred over 600 million years ago, left no evidence in the fossil record. To trace the earliest events in animal prehistory, we compare extant animals to their closest living relatives, the choanoflagellates, in order to reconstruct the gene content of their last common ancestor and how it evolved on the stem lineage leading to animals. In this project, we increase the accuracy of ancestral animal and choanoflagellate gene content reconstructions by sequencing the transcriptomes of 19 species of choanoflagellates selected for their phylogenetic diversity.

Associated Publications:

Richter, Daniel J and Fozouni, Parinaz and Eisen, Michael and King, Nicole. Gene family innovation, conservation and loss on the animal stem lineage. 2018;7:e34226 https://doi.org/10.7554/eLife.34226

Affiliations: Station Biologique de Roscoff, Adaptation et Diversité en Milieu Marin - UMR 7144 CNRS UPMC, University of California, San Francisco, University of California, Berkeley, University of California, Berkeley

External URL: http://kinglab.berkeley.edu/

Version: 1

Publication Date: 2017

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Source: Protocols.io