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Jernej Turnsek, Pardis Gholami 2017. Guidelines for highly efficient construction of diatom episomes using Gibson Assembly . protocols.io dx.doi.org/10.17504/protocols.io.jy7cpznCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.jy7cpzn
Authors: Jernej Turnsek, Pardis Gholami
Group: Protist Research to Optimize Tools in Genetics (PROT-G)
Summary: This protocol presents a Gibson Assembly design for highly efficient construction of diatom episomes. We regularly observe >90% efficiency (efficiency = % of screened bacterial colonies containing the desired construct) following the steps presented here.
Affiliations: University of California, Berkeley, UC San Diego, Scripps Institution of Oceanography (SIO) | J. Craig Venter Institute (JCVI)
Version: 1
Publication Date: 2017
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Source: Protocols.io
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