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URL: https://dx.doi.org/10.17504/protocols.io.jxicpke

Authors: Marie-Ka Tilak

Summary: This protocol was developed with the aim of sequencing the GC rich part of heterogeneous genomes with the Illumina technology. To this aim, fragmented genomic DNA is denaturized up to 90°C before library preparation. This procedure enriches the library in GC-rich fragments and leads to a substantial increase in average GC content of sequence reads.

Associated Publications: Tilak M, Botero-Castro F, Galtier N, Nabholz B, Illumina Library Preparation for Sequencing the GC-Rich Fraction of Heterogeneous Genomic DNA. Genome Biology and Evolution 10(2). doi: 10.1093/gbe/evy022

Affiliations: 1 Institut des Sciences de l'Evolution de Montpellier (ISE-M) UMR 5554 Université de Montpellier - Centre National de la Recherche Scientifique - Institut de Recherche pour le Développement – École Pratique des Hautes Études

External URL: https://doi.org/10.1093/gbe/evy022

Version: 1

Publication Date: 2017

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