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URL: https://dx.doi.org/DOI:10.17504/protocols.io.ifccbiw

Authors: Gomard Yann, Lagadec Erwan and Tortosa Pablo

Summary: This protocol is adapted from that detailed in the International Course on Laboratory methods for the Diagnosis of Leptospirosis, by Dr. R.A. Hartskeerl, Dr. H.L. Smits, Mr. H. Korver, Dr. M.G.A. Goris and Dr. W.J. Terpstra from the Leptospirosis Reference Center in Amsterdam, the Netherlands. It is routinely used at UMR PIMIT, St Denis de La Réunion, France, for the isolation of pathogenic Leptospira from wild animals (terrestrial small mammals, bats and dogs). For Leptospira isolation from urine, go directly to step 10.

Associated Publications: Biscornet L, Dellagi K, Pagès F, Bibi J, Comarmond Jd, Mélade J, Govinden G, Tirant M, Gomard Y, Guernier V, Lagadec E, Mélanie J, Rocamora G, Minter GL, Jaubert J, Mavingui P, Tortosa P (2017) Human leptospirosis in Seychelles: A prospective study confirms the heavy burden of the disease but suggests that rats are not the main reservoir. PLoS Negl Trop Dis 11(8): e0005831. doi: 10.1371/journal.pntd.0005831

Affiliations: UMR PIMIT (Processus Infectieux en Milieu Insulaire Tropical) Université de La Réunion, CNRS 9192, INSERM 1187, IRD 249 , UMR PIMIT (Processus Infectieux en Milieu Insulaire Tropical) Université de La Réunion, CNRS 9192, INSERM 1187, IRD 249

External URL: https://doi.org/10.1371/journal.pntd.0005831

Version: 1

Publication Date: 2017

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