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Protocol Name
Immunofluorescence: EGFR, ICAM-1 and FAS detection
DOI:10.17504/protocols.io.hwgb7bw RRID Copied  
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Sandra L. Ross,  Marika Sherman,  Patricia L. McElroy,  Julie A. Lofgren,  Gordon Moody,  Patrick A. Baeuerle,  Angela Coxon,  Tara Arvedson 2017. Immunofluorescence:  EGFR, ICAM-1 and FAS detection. protocols.io dx.doi.org/10.17504/protocols.io.hwgb7bw
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.hwgb7bw

Authors: Sandra L. Ross, Marika Sherman, Patricia L. McElroy, Julie A. Lofgren, Gordon Moody, Patrick A. Baeuerle, Angela Coxon, Tara Arvedson

Summary: This protocol describes methods for detecting cell-surface markers by indirect immunofluorescence after treatment with BiTE®. Cells are analyzed by cellular imaging.

Associated Publications: Ross SL, Sherman M, McElroy PL, Lofgren JA, Moody G, Baeuerle PA, Coxon A, Arvedson T, Bispecific T cell engager (BiTE) antibody constructs can mediate bystander tumor cell killing. PLoS ONE 12(8). doi: 10.1371/journal.pone.0183390

Affiliations: Department of Oncology Research, Amgen Inc., Department of Oncology Research, Amgen Inc., Department of Oncology Research, Amgen Inc., Department of Oncology Research, Amgen Inc., Department of Oncology Research, Amgen Inc., Department of Oncology Research, Amgen Inc., Department of Oncology Research, Amgen Inc., Department of Oncology Research, Amgen Inc.

External URL: https://doi.org/10.1371/journal.pone.0183390

Version: 1

Publication Date: 2017

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Source: Protocols.io