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Mark Dewitt & Julia Wong 2017. Cas9 RNP nucleofection for cell lines using Lonza 4D Nucleofector. protocols.io dx.doi.org/10.17504/protocols.io.hd8b29wCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.hd8b29w
Authors: Mark Dewitt & Julia Wong
Group: Innovative Genomics Institute, CornLab
Summary: This protocol, based on published work, demonstrates how to delivery Cas9 RNP-based gene editing reagents to cultured mamallian cells by electroporation with a Lonza 4d Nucleofector. Consider consulting some of the following papers:1. RNP delivery paper upon which this work is based (Open Access):https://elifesciences.org/content/3/e047662. Paper by an IGI post-doc that details the rationale behind HDR donor design:https://www.ncbi.nlm.nih.gov/pubmed/26789497
Version: 3
Publication Date: 2017
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Source: Protocols.io
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