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Protocol Name
Preparation of chemically competent E. coli cells
DOI:10.17504/protocols.io.gtcbwiw RRID Copied  
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Alice Pawlowski 2020. Preparation of chemically competent E. coli cells. protocols.io dx.doi.org/10.17504/protocols.io.gtcbwiw
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.gtcbwiw

Authors: Alice Pawlowski

Summary: Standard protocol to prepare cells for highly efficient DNA uptake using CaCl2 (Sambrook and Russell, Molecular Cloning: A Laboratory Manual, 2001).

Affiliations: Institute of Synthetic Microbiology, Heinrich Heine University Düsseldorf

Version: 1

Publication Date: 2020

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Source: Protocols.io