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Protocol Name
DOI:10.17504/protocols.io.fnnbmde RRID Copied  
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Darach Miller 2016. scratch, don't use, delete ( the biology is unexpected and something we don't want to invest time in figuring down right now ). protocols.io dx.doi.org/10.17504/protocols.io.fnnbmde
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.fnnbmde

Authors: Darach Miller

Summary: The aim of this protocol is to describe how to do a very reproducible upshift of a nitrogen-limited yeast culture to nitrogen non-limited conditions. In order to do this with enough cells to make inefficient analysis (ie RATEseq) feasible, we start in the chemostat for high-cell counts.

Version: 2

Publication Date: 2016

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Source: Protocols.io