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Sarah Giuliani 2016. Sampling for qEXT and MPN assays: Large-scale One-step Phage Infection of Cyanobacteria. protocols.io dx.doi.org/10.17504/protocols.io.fh3bj8nCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.fh3bj8n
Authors: Sarah Giuliani
Group: Coleman Lab
Summary: 15 Hourly Timepoints: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14Sampling is for qPCR assay to determine total extracellular phage (qEXT) and MPN (most probable number) assay to determine % phage infectivity.For qEXT and MPN sampling, 250 µL of sample in duplicates were collected and filtered through a 0.2 µm, 96-well filter plate, for each time point. (Flow through filtrate containing phage is the sample to analyze).
Affiliations: Coleman Lab - University of Chicago, Department of the Geophysical Sciences
Version: 1
Publication Date: 2016
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Source: Protocols.io
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