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Amy Zimmerman 2016. Nutrient deplete/replete algal culture for elemental analysis. protocols.io dx.doi.org/10.17504/protocols.io.fcdbis6Copy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.fcdbis6
Authors: Amy Zimmerman
Group: VERVE Net, Worden Lab
Summary: Purpose: To evaluate the influence of nutrient availability on cellular elemental composition (quotas and stoichiometry) in marine picoeukaryotes.Elemental quotas and ratios are assessed under nutrient replete and deplete conditions at the same time to minimize potential variation between experiments. Cells from an exponentially growing culture are concentrated by centrifugation, washed and re-suspended in a small volume of nutrient deplete media. These concentrated cells are used to inoculate triplicate replete and deplete culture flasks at a starting density corresponding to early-exponential growth. Culture growth is monitored daily. When the mean growth rate of the nutrient deplete treatment is half or less of the replete treatment (GRDEP/GRREP
Affiliations: Monterey Bay Aquarium Research Institute
Version: 1
Publication Date: 2016
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Source: Protocols.io
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