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URL: https://dx.doi.org/10.17504/protocols.io.e7ebhje

Authors: Kelsey Miller

Group: BioLegend

Summary: The HLA class I ELISA is an enzyme immunoassay based on the detection of β2-microglobulin subunit of HLA class I complexes, after capturing the complex through the conjugated biotin. To this end, biotinylated HLA class I complex is first captured in streptavidin coated microtiter wells. Subsequently, HRP-conjugated anti-human β2-microglobulin is added to detect intact HLA class I complexes. Only intact HLA class I complexes are recognized. Peptides with high affinity binding will be clearly detected by this ELISA technique, while peptides with a moderate to low binding affinity for HLA class I provide a moderate to non-detectable signal. This protocol is designed to evaluate the efficiency of peptide exchange when using the Flex-T™ system.

Affiliations: BioLegend

External URL: http://www.biolegend.com/media_assets/support_protocol/Protocol%20for%20HLA%20class%20I%20ELISA%2006202016.pdf

Version: 1

Publication Date: 2016

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