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Protocol Name
DOI:10.17504/protocols.io.cikucv RRID Copied  
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Sean Seaver 2014. Blasticidin S HCl in Mammalian Culture Determine Working Concentration. protocols.io dx.doi.org/10.17504/protocols.io.cikucv
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.cikucv

Authors: Sean Seaver

Summary: The working concentration of blasticidin S HCl for use in mammalian cell gene selection can range anywhere from 2-100μg/mL depending on the cell lines used.Optimal doses typically range from 2-20μg/mLHowever, we recommend performing a kill curve to determine the optimal dose for your cell lines.

Affiliations: P212121

External URL: http://store.p212121.com/blasticidin-s-hcl/

Version: 1

Publication Date: 2014

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Data and Source Information

Source: Protocols.io