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Protocol Name
Manual H&E Staining
DOI:10.17504/protocols.io.bsi8nchw RRID Copied  
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Jia Ren Lin 2021. Manual H&E Staining. protocols.io dx.doi.org/10.17504/protocols.io.bsi8nchw
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.bsi8nchw

Authors: Jia Ren Lin

Group: Laboratory of Systems Pharmacology, NCIHTAN

Summary: The hematoxylin and eosin (H&E) stain is the standard used for microscopic examination of fixed tissue specimens. This procedure establishes a consistent process for the manual preparation of H&E-stained slides from FFPE tissue specimens. H&E staining is compatible with, and frequently performed after tissue-based cyclic immunofluorescence (t-CyCIF), enabling conventional histopathology review to be combined with fluorescence imaging.

Affiliations: Harvard Medical School, Laboratory of Systems Pharmacology

Version: 1

Publication Date: 2021

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Source: Protocols.io