Resource Summary Report  New Search Previous Search Results

URL: https://dx.doi.org/10.17504/protocols.io.bqvrmw56

Authors: Joost Mansour, Fabrice Not

Group: Ecology of Marine Plankton (ECOMAP) team - Roscoff, Symbiosis Model Systems

Summary: Many marine protists are not culturable and therefore challenging to study, nonetheless, they are essential in all marine ecosystems. The development of single-cell techniques is allowing for more marine protists to be studied. Thereby Cultivation-independent studies are essential for the study of organisms sensitive to the sampling procedure, such as Radiolaria.This method describes the means to acquire clean isolated single-cells from plankton nets for physiological and/or transcriptomic analysis. The isolation of cells in this protocol is followed by preparing the cells for RNA extraction (protocol: Single-cell total RNA extraction from marine protists (e.g. Acantharia, Strombidium cf basimorphum, and Prymnesium parvum)).

Affiliations: Sorbonne University, CNRS, UMR7144 Adaptation and Diversity in Marine Environment (AD2M) laboratory, Ecology of Marine Plankton team, Station Biologique de Roscoff, Place Georges Teissier, 29680 Roscoff, France, Sorbonne University, CNRS, UMR7144 Adaptation and Diversity in Marine Environment (AD2M) laboratory, Ecology of Marine Plankton team, Station Biologique de Roscoff, Place Georges Teissier, 29680 Roscoff, France

Version: 2

Publication Date: 2021

Expand All Collapse