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URL: https://dx.doi.org/DOI:10.17504/protocols.io.bqevmte6

Authors: Katy Parker, Matt Bristow

Summary: There has been considerable interest in the measurement of cortisol in hair as a biomarker of stress since work by Davenport et al. (2006). The methodology developed by our laboratory is intended to allow for a scalable method for hair analysis that can be undertaken for large numbers of hair samples and has the minimum number of steps to reduce the chance of error and reduce costs. Our method reduces the number of steps where the sample is transferred to another container as it is kept in the same tube from initial weighing through to end of the methanol incubation. The procedure is also very scalable. The ‘rate limiting’ step for our hair analysis procedure is the grinding of the hair and this has been partially automated using the MP Biological Fast Prep grinder which is highly scalable. Final quantification of cortisol is performed via an ELISA assay, an assay which is rapid and cost effective.References:Davenport, M. D., Tiefenbacher, S., Lutz, C. K., Novak, M. a., & Meyer, J. S. (2006). Analysis of endogenous cortisol concentrations in the hair of rhesus macaques. General and Comparative Endocrinology, 147(3), 255–261. https://doi.org/10.1016/j.ygcen.2006.01.005

Affiliations: ARU Biomarker Laboratory, Anglia Ruskin University, ARU Biomarker Laboratory, Anglia Ruskin University

Version: 1

Publication Date: 2020

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