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URL: https://dx.doi.org/10.17504/protocols.io.bji2kkge

Authors: Jia Ren Lin, Benjamin Izar, Zoltan Maliga, Yu-An Chen, Giorgio Gaglia, Ziming Du, Clarence Yapp, Shaolin Mei, Sandro Santagata, Peter Sorger

Group: Laboratory of Systems Pharmacology, NCIHTAN

Summary: Tissue-based cyclic immunofluorescence (t-CyCIF) is optimized for FFPE specimens mounted on glass slides. Dewaxing and antigen retrieval are important steps to remove wax and expose antigenic sites. This protocol describes dewaxing and antigen retrieval on a Leica Bond RX automated slide processor; similar instruments are manufactured by Ventana or Dako and are commonly found in histopathology core facilities. t-CyCIF can also be performed following manual de-waxing and antigen retrieval (e.g. microwaving slides in citrate buffer or using a pressure cooker).

Affiliations: Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA, Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA;Ludwig Center for Cancer Research at Harvard, Harvard Medical School, Boston, MA, Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA;Ludwig Center for Cancer Research at Harvard, Harvard Medical School, Boston, MA, Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA, Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA;Ludwig Center for Cancer Research at Harvard, Harvard Medical School, Boston, MA;Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA;Ludwig Center for Cancer Research at Harvard, Harvard Medical School, Boston, MA, Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA, Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA, Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA;Ludwig Center for Cancer Research at Harvard, Harvard Medical School, Boston, MA;Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA;Ludwig Center for Cancer Research at Harvard, Harvard Medical School, Boston, MA

External URL: www.cycif.org

Version: 2

Publication Date: 2020

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