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URL: https://dx.doi.org/10.17504/protocols.io.biwfkfbn

Authors: Oscar Whitney, Basem Al-Shayeb, Alex Crits-Cristoph, Mira Chaplin, Vinson Fan, Hannah Greenwald, Adrian Hinkle, Rose Kantor, Lauren Kennedy, Anna Maurer, Robert Tjian, Kara L. Nelson, UC Berkeley Wastewater-based epidemiology consortium

Group: Coronavirus Method Development Community

Summary: The following protocol describes the "4S" (Sewage, Salt, Silica and SARS-CoV-2) workflow applied to using dry silica powder as an RNA-binding matrix instead of silica spin columns. This offers an even more economical alternative, requiring only centrifugation to extract RNA from wastewater. This procedure is intended to be carried out in a BSL2+ laboratory space, with precautions when handling raw wastewater samples.

Affiliations: University of California, Berkeley, Tjian & Darzacq laboratory, University of California, Berkeley, Banfield & Doudna laboratory, University of California, Berkeley, Banfield laboratory, University of California, Berkeley, Nelson laboratory, University of California, Berkeley, Tjian & Darzacq laboratory, University of California, Berkeley, Nelson laboratory, University of California, Berkeley, Nelson laboratory, University of California, Berkeley, Nelson laboratory, University of California, Berkeley, Nelson laboratory, University of California, Berkeley, Tjian & Darzacq laboratory, University of California, Berkeley, HHMI, University of California, Berkeley, University of California, Berkeley

Version: 1

Publication Date: 2020

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