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Aaron Bivins, Warish Ahmed, Devin North, Kyle Bibby 2020. Wastewater Concentration by Adsorption and Direct Extraction for SARS-CoV-2 RNA Detection and Quantification using RT-ddPCR. protocols.io dx.doi.org/10.17504/protocols.io.bhiuj4ewCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bhiuj4ew
Authors: Aaron Bivins, Warish Ahmed, Devin North, Kyle Bibby
Group: Coronavirus Method Development Community, 2019-nCoV Wastewater Epidemiology
Summary: The following protocol describes the GERM Lab workflow for processing wastewater for SARS-CoV-2 via pH adjustment, MCE filtration, extraction, and assay by droplet digital PCR (ddPCR). It is synonymous to method A as described in Ahmed et al. 2020 (the referenced manuscript) with modifications to the consumables and PCR format. It is intended for use in a BSL2+ lab with extra precautions for processing environmental specimens for SARS-CoV-2 as described in the CDC Interim Laboratory Biosafety Guidelines.
Affiliations: University of Notre Dame, CSIRO, University of Notre Dame, University of Notre Dame
External URL: https://www.sciencedirect.com/science/article/pii/S004896972033480X
Version: 2
Publication Date: 2020
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Source: Protocols.io
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