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Mark Dewitt, Julia Wong, Beeke Wienert 2020. In vitro transcription of guide RNAs and 5'-triphosphate removal. protocols.io dx.doi.org/10.17504/protocols.io.bfxfjpjnCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bfxfjpjn
Authors: Mark Dewitt, Julia Wong, Beeke Wienert
Group: CornLab
Summary: sgRNA template assembly, in vitro T7 transcription, and sgRNA column cleanup to remove 5'-triphosphate groups
Affiliations: UC BERKELEY-IGI, UC BERKELEY-IGI, UC BERKELEY-IGI
External URL: https://doi.org/10.1371/journal.pbio.2005840
Version: 11
Publication Date: 2020
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Source: Protocols.io
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