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Maja Rennig, Kristoffer Bach Falkenberg, Cristina Hernandez Rollan, Andreas Birk Bertelsen, Morten Norholm 2020. Protein expression on the surface of Escherichia coli. protocols.io dx.doi.org/10.17504/protocols.io.bfh4jj8wCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bfh4jj8w
Authors: Maja Rennig, Kristoffer Bach Falkenberg, Cristina Hernandez Rollan, Andreas Birk Bertelsen, Morten Norholm
Summary: Display of proteins on the bacterial cell surface has always been an attractive technique for the production of functional cell-anchored proteins, thereby reducing the cost, time and effort related to enzyme purification. Thus, surface display can enable fast and easy screening of protein libraries, e.g. activity variants, or screening of different enzyme substrates, while maintaining a connection between the phenotype and the genotype. Additionally, cells displaying functional enzymes can potentially be used as whole-cell catalysts.This protocol describes how to express proteins on the surface of E. coli BL21 (DE3) using a L-rhamnose inducible expression system.
Affiliations: Technical University of Denmark, Technical University of Denmark, Technical University of Denmark, Technical University of Denmark, Technical University of Denmark
Version: 1
Publication Date: 2020
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Source: Protocols.io
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