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Protocol Name
DOI:10.17504/protocols.io.bffvjjn6 RRID Copied  
PDF Report How to cite
Franchesca Farris, Maigan Brusko 2020. HuBMAP UF TMC - Single Cell RNA Seq (10x Genomics) Library Preparation and Sequencing . protocols.io dx.doi.org/10.17504/protocols.io.bffvjjn6
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.bffvjjn6

Authors: Franchesca Farris, Maigan Brusko

Group: Human BioMolecular Atlas Program (HuBMAP) Method Development Community

Summary: This protocol is for the generation of 3' (chemistry version 3) cDNA libraries from individual cells via droplet generation utilizing the 10x Genomics Chromium Controller and associated reagents. Paired end libraries are then sequenced on Illumina instruments.

Affiliations: University of Florida, University of Florida

Version: 1

Publication Date: 2020

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Source: Protocols.io