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Maja Rennig, Kristoffer Bach Falkenberg, Cristina Hernandez Rollan, Andreas Birk Bertelsen, Morten Norholm 2020. Protein expression in Komagataella phaffii (formerly Pichia pastoris). protocols.io https://dx.doi.org/10.17504/protocols.io.bd32i8qeCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bd32i8qe
Authors: Maja Rennig, Kristoffer Bach Falkenberg, Cristina Hernandez Rollan, Andreas Birk Bertelsen, Morten Norholm
Summary: The methylotrophic yeast Komagataella phaffii (formerly Pichia pastoris) is the most commonly used yeast species in the production of recombinant proteins. This is most likely due to its ability to grow to high cell density, to express recombinant genes in a tightly controlled manner and to efficiently secrete proteins. Despite its biotechnological importance and wide use in industry, relatively few genetic tools are readily available for academic research. Here, we present a protocol for production of proteins in K. phaffii.The protocol found here is modified from the protocol provided in the Pichia Expression Kit (Catalog Number K1710-01, Invitrogen).
Affiliations: Technical University of Denmark, Technical University of Denmark, Technical University of Denmark, Technical University of Denmark, Technical University of Denmark
Version: 1
Publication Date: 2020
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