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Allen Institute for Brain Science 2020. PCR Prep from cDNA for IVT Reactions. protocols.io dx.doi.org/10.17504/protocols.io.bcsfiwbnCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bcsfiwbn
Authors: Allen Institute for Brain Science
Group: BICCN, Allen Institute for Brain Science
Summary: This protocol describes PCR1 and PCR2 reactions beginning with cDNA to generate transcript templates ready for IVT reaction. This protocol is written for 192 (2x96) reactions but can also be used for fewer or more reactions by adjusting the amount of materials and equipment appropriately.
Affiliations: Allen Institute
Version: 1
Publication Date: 2020
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Source: Protocols.io
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