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URL: https://dx.doi.org/10.17504/protocols.io.bbwaipae

Authors: Sam Li

Group: BioLegend

Summary: BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed.

Affiliations: BioLegend

External URL: https://www.biolegend.com/protocols/mojosort-human-cd14--monocyte-isolation-kit-column-protocol/4764/

Version: 2

Publication Date: 2020

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