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Jeffrey Lewis, Amanda Scholes 2020. Quantitative (q)PCR and Differential Expression Analysis. protocols.io dx.doi.org/10.17504/protocols.io.bbgpijvnCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bbgpijvn
Authors: Jeffrey Lewis, Amanda Scholes
Group: Protist Research to Optimize Tools in Genetics (PROT-G), Lewis Lab
Summary: A generalizable protocol for measuring relative changes in gene expression via qPCR (cDNA synthesis, qPCR primer optimization, and qPCR analysis), with specific optimization for the budding yeast Saccharomyces cerevisiae.
Associated Publications: Scholes AN, Lewis JA, Comparison of RNA isolation methods on RNA-Seq: implications for differential expression and meta-analyses. BMC Genomics doi: 10.1186/s12864-020-6673-2
Affiliations: University of Arkansas - Fayetteville, University of Arkansas - Fayetteville
External URL: https://doi.org/10.1186/s12864-020-6673-2
Version: 1
Publication Date: 2020
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Source: Protocols.io
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