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Protocol Name
HuBMAP - Tissue Sectioning for CODEX Specimens
DOI:10.17504/protocols.io.basdiea6 RRID Copied  
PDF Report How to cite
Leigh Propper, Marda Jorgensen 2019. HuBMAP - Tissue Sectioning for CODEX Specimens. protocols.io dx.doi.org/10.17504/protocols.io.basdiea6
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.basdiea6

Authors: Leigh Propper, Marda Jorgensen

Group: Human BioMolecular Atlas Program (HuBMAP) Method Development Community

Summary: This method details the microtomy process for specimens that will be stained and analyzed using the Akoya CODEX® system.This process applies to paraffin embedded blocks containing tissue of no more than 1cm x 1cm in size.FFPE tissues for CODEX® analysis must be sectioned onto prepared poly-l-lysine coated coverslips.Cut and mounted tissue sections can be stored at 4°C for up to one(1 )month prior to staining.

Affiliations: University of Florida, University of Florida

Version: 2

Publication Date: 2019

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Source: Protocols.io