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Janet Keast, Peregrine Osborne 2020. Light microscopic analysis of synaptic input to neurons in the rat major pelvic ganglion [keast-003]. protocols.io dx.doi.org/10.17504/protocols.io.bakiicueCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bakiicue
Authors: Janet Keast, Peregrine Osborne
Group: SPARC
Summary: This collection describes the procedures required to visualise and characterize synaptic boutons associated with functionally classified pelvic ganglion autonomic neurons of adult male and female Sprague-Dawley rats. This collection includes protocols for:STAGE 1: Surgery to micro-inject fluorescent retrograde tracer dyes into one or more sites within the lower urinary tractSTAGE 2: Intracardiac perfusion with fixative to preserve neural tissues of interestSTAGE 3: Immunohistochemical labeling of thick cryosections of pelvic gangliaSTAGE 4: Confocal microscopy and image analysis of synaptic boutons associated with ganglion neurons.
Affiliations: University of Melbourne, University of Melbourne
Version: 1
Publication Date: 2020
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Source: Protocols.io
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