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URL: https://dx.doi.org/10.17504/protocols.io.9j5h4q6

Authors: Stefano Vianello, Mehmet Girgin, Giuliana Rossi, Matthias Lutolf

Summary: Gastruloid generation protocol as performed in the Lutolf Lab, EPFL.For previously published protocols, see:Baillie-Johnson, Peter, et al. "Generation of aggregates of mouse embryonic stem cells that show symmetry breaking, polarization and emergent collective behaviour in vitro."JoVE (Journal of Visualized Experiments)105 (2015): e53252. andMehmet, G., et al. "Generating Gastruloids from Mouse Embryonic Stem Cells."Protocol Exchange (2018).

Affiliations: Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences (SV) and School of Engineering (STI), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland,, Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences (SV) and School of Engineering (STI), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland,, Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences (SV) and School of Engineering (STI), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland,, Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences (SV) and School of Engineering (STI), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland and Institute of Chemical Sciences and Engineering, School of Basic Science (SB), EPFL, Lausanne, Switzerland

Version: 1

Publication Date: 2020

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