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Linda Silvestri, Eftychis Frangedakis, Susana Sauret-Gueto, Marius Rebmann, Marta Tomaselli 2019. Marchantia agrobacterium transformation of sporelings in multi-well plates (plus materials info). protocols.io dx.doi.org/10.17504/protocols.io.92ah8aeCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.92ah8ae
Authors: Linda Silvestri, Eftychis Frangedakis, Susana Sauret-Gueto, Marius Rebmann, Marta Tomaselli
Group: OpenPlant Project
Summary: A modification of the Ishizaki et al 2008 Agrobacterium mediated Marchantia sporeling transformation protocol is used. Sterilised spores are grown for 5 days in 0.5x strength Gamborg plates prior to co-cultivation for 2 days with agrobacterium in liquid media in multiwell plates. Sporelings are then spread on media with the appropriate selective antibiotic. In about 7 days, positive transformants start to emerge.
Affiliations: Plant Sciences, University of Cambridge, OpenPlant, University of Cambridge, Plant Sciences, University of Cambridge, OpenPlant, Plant Sciences, University of Cambridge, OpenPlant, Plant Sciences, University of Cambridge, OpenPlant
Version: 2
Publication Date: 2019
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Source: Protocols.io
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