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E. Dale Abel 2019. Evaluation of Mitochondrial Function. protocols.io https://dx.doi.org/10.17504/protocols.io.7s8hnhwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.7s8hnhw
Authors: E. Dale Abel
Group: Diabetic Complications Consortium
Summary: This protocol describes the procedures used by the DiaComp for evaluating mitochondrial function.Diabetic Complication:References 1. Veksler VI, Kuznetsov AV, Sharov VG, Kapelko VI, Saks VA. Mitochondrial respiratory parameters in cardiac tissue: a novel method of assessment by using saponin-skinned fibers. Biochim Biophys Acta. 1987;892:191-6. 2. Saks VA, Veksler VI, Kuznetsov AV, Kay L, Sikk P, Tiivel T, Tranqui L, Olivares J, Winkler K, Wiedemann F, Kunz WS. Permeabilized cell and skinned fiber techniques in studies of mitochondrial function in vivo. Mol Cell Biochem. 1998;184:81-100. 3. Ouhabi R, Boue-Grabot M, Mazat JP. Mitochondrial ATP synthesis in permeabilized cells: assessment of the ATP/O values in situ. Anal Biochem. 1998;263:169-75.
Affiliations: University of Iowa
External URL: https://www.diacomp.org/shared/document.aspx?id=42&docType=Protocol
Version: 2
Publication Date: 2019
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