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Protocol Name
DOI:10.17504/protocols.io.7fthjnn RRID Copied  
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Ruben M Costa, Anny Cárdenas, Christian Voolstra 2019. Protocol for bacterial depletion of Aiptasia anemones - Towards the generation of gnotobiotic/germ-free cnidarian host animals. protocols.io dx.doi.org/10.17504/protocols.io.7fthjnn
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.7fthjnn

Authors: Ruben M Costa, Anny Cárdenas, Christian Voolstra

Group: reefgenomics, Aiptasia-Symbiodiniaceae Model System

Summary: This protocol aims to obtain bacteria-depleted Aiptasia polyps. It is divided into 2 sections: (1) a rearing protocol composed of sterile food preparation and anemone priming and (2) a bacteria-depletion protocol for Aiptasia.

Affiliations: Red Sea Research Center, Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia, Department of Biology, University of Konstanz, 78457 Konstanz, Germany, Department of Biology, University of Konstanz, 78457 Konstanz, Germany

Version: 1

Publication Date: 2019

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Source: Protocols.io