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Alba Balletbó 2019. Gel Electrophoresis. protocols.io dx.doi.org/10.17504/protocols.io.7eehjbeCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.7eehjbe
Authors: Alba Balletbó
Group: iGEM Wageningen 2019
Summary: Gel electrophoresis is the standard lab procedure for separating DNA by size (e.g., length in base pairs) for visualization and purification. Electrophoresis uses an electrical field to move the negatively charged DNA through an agarose gel matrix toward a positive electrode. Shorter DNA fragments migrate through the gel more quickly than longer ones. Thus, you can determine the approximate length of a DNA fragment by running it on an agarose gel alongside a DNA ladder (a collection of DNA fragments of known lengths).
Affiliations: Wageningen University
Version: 1
Publication Date: 2019
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Source: Protocols.io
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