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Marcos Otero-Garcia, Inma Cobos 2019. Isolation of single somas from postmortem fresh frozen human brain and immunostaining for AT8 and MAP2. protocols.io https://dx.doi.org/10.17504/protocols.io.6uqhevwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.6uqhevw
Authors: Marcos Otero-Garcia, Inma Cobos
Group: Neurodegeneration Method Development Community
Summary: - Protocol optimized to isolate single neuronal somas with cytoplasmic protein aggregates from postmortem fresh frozen brain- Works for the isolation of neuronal somas with neurofibrillary tangles from Alzheimer disease's brains- Note that cell membranes are highly disrupted in tissues that were previously frozen. Thus, cytoplasmic transcripts are largely lost. This protocol is therefore useful for the isolation of specific cell populations using antibodies against cytoplasmic antigens, but not for the profiling of cytoplasmic mRNA.
Affiliations: Stanford University, Stanford
Version: 1
Publication Date: 2019
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