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URL: https://dx.doi.org/DOI:10.17504/protocols.io.54jg8un

Authors: Eliana Rocio Rodríguez Gómez, William Otero Regino, Pedro A. Monterrey, Alba Alicia Trespalacios Rangel

Summary: In this study , we aimed to determine the current circulation of cagA gene EPIYA motifs present in Colombian Helicobacter pylori isolates using a rapid molecular test. The cagA gene 3' region was amplified through conventional Polymerase Chain Reaction (PCR) and PCR products obtained were sequenced and analyzed with bioinformatics tools.Aditionally, to confirm the prediction of the number EPIYA C repeats based on the PCR product molecular weight, reamplification and secuencing analysis were performed.

Associated Publications: Gómez ERR, Regino WO, Monterrey PA, Rangel AAT (2020) cagA gene EPIYA motif genetic characterization from Colombian Helicobacter pylori isolates: Standardization of a molecular test for rapid clinical laboratory detection. PLoS ONE 15(1): e0227275. doi: 10.1371/journal.pone.0227275

Affiliations: Microbiology Department, Pontificia Universidad Javeriana, Bogotá, Colombia, Gastroenterology Unit, Clínica Fundadores, Bogotá, Colombia, Natural Sciences and Mathematics Faculty, Universidad del Rosario, Bogotá, Colombia., Microbiology Department, Pontificia Universidad Javeriana, Bogotá, Colombia

External URL: https://doi.org/10.1371/journal.pone.0227275

Version: 1

Publication Date: 2019

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