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David Eccles 2019. Preparing Reads for Stranded Mapping. protocols.io https://dx.doi.org/10.17504/protocols.io.43qgymwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.43qgymw
Authors: David Eccles
Summary: This protocol is for preparing long reads for stranded mapping, as an intermediate step for additional protocols:Aligning strand-oriented sequences to a transcriptome for transcript / gene countingAligning strand-oriented sequences to a genome for confirmatory QCInput(s): demultiplexed fastq files (see protocol Demultiplexing Nanopore reads with LAST), adapter file (containing strand-sensitive adapter sequences)Output(s): oriented read files, as gzipped fastq files
Affiliations: Malaghan Institute of Medical Research (NZ)
Version: 4
Publication Date: 2019
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