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Akila Wijerathna Yapa, Andrew Bowerman, Diep Ganguly 2019. RNA extractions from de-etiolated Arabidopsis seedlings using CTAB. protocols.io dx.doi.org/10.17504/protocols.io.3f6gjreCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.3f6gjre
Authors: Akila Wijerathna Yapa, Andrew Bowerman, Diep Ganguly
Group: Pogson Genomics Group, EBL_ANU
Summary: A CTAB based method for extracting RNA, which is particularly useful for tough tissues. This has been adapted from a protocol that was originally used for pine tree tissue, which is difficult due to the high concentrations of polysaccharides, phenolics, and RNase (Chang, S., Puryear, J. & Cairney, J. Plant Mol Biol Rep (1993) 11: 113. https://doi.org/10.1007/BF02670468). The protocol described herein was an effective alternative to TRIzol based extractions for recovering RNA from juvenile de-etiolated tissues.
Affiliations: The Hebrew University of Jerusalem , The Australian National University, The Australian National University
Version: 1
Publication Date: 2019
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Source: Protocols.io
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