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Sandra Rieger 2019. Quantitative PCR analysis to assess gene expression changes in hyperglycemic larval zebrafish. protocols.io dx.doi.org/10.17504/protocols.io.3bagiieCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.3bagiie
Authors: Sandra Rieger
Group: Diabetic Complications Consortium
Summary: To assess hyperglycemia in larval zebrafish, we analyzed the expression of genes implicated in glucose metabolism, such as insulin, insulin receptor, glucagon, and phosphoenolpyruvate carboxylase (pepck), using quantitative PCR (qPCR). We analyzed gene expression following treatment of Tg(ins:NTRmCherry) transgenic larvae either with 0.5 % DMSO (controls) or 10 mM metronidazole (see Protocol 2) for β-cell ablation at 3 and 8 days post fertilization (dpf). Diabetic Complication:
Affiliations: University of Miami
External URL: https://www.diacomp.org/shared/document.aspx?id=222&docType=Protocol
Version: 1
Publication Date: 2019
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Source: Protocols.io
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