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Timothy Gilpatrick, Isac Lee, James E. Graham, Etienne Raimondeau, Rebecca Bowen, Andrew Heron, Fritz J. Sedlazeck, Winston Timp 2019. Cas9 Enrichment for Nanopore Sequencing. protocols.io dx.doi.org/10.17504/protocols.io.3acgiawCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.3acgiaw
Authors: Timothy Gilpatrick, Isac Lee, James E. Graham, Etienne Raimondeau, Rebecca Bowen, Andrew Heron, Fritz J. Sedlazeck, Winston Timp
Summary: This protocol is designed to help users achieve targeted enrichment for regions of interest using nanopore sequencing, providing higher coverage for myriad future analysis applications. GuideRNA components (crRNA and tracrRNA) were designed using online tools provided by IDT.
Affiliations: Johns Hopkins University, Johns Hopkins University, Oxford Nanopore Technologies, Oxford Nanopore Technologies, Oxford Nanopore Technologies, Oxford Nanopore Technologies, Baylor College of Medicine, Johns Hopkins University
Version: 1
Publication Date: 2019
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Source: Protocols.io
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