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Elin Einarsson 2019. Golden Gate plasmids used for transfection of Perkinsus marinus. protocols.io dx.doi.org/10.17504/protocols.io.37egrjeCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.37egrje
Authors: Elin Einarsson
Group: Protist Research to Optimize Tools in Genetics (PROT-G)
Summary: We have created plasmids for transfection of the parasite Perkinsus marinus using Golden Gate cloning. We have explored the possibility to either FACS sort (GFP/mCherry fused to MOE membrane protein) or using drug selection (bleomycin, blasticidin S or puromycin) to enrich transfected cells.
Affiliations: Waller Lab, Department of Biochemistry, University of Cambridge
Version: 1
Publication Date: 2019
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Source: Protocols.io
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