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URL: https://dx.doi.org/10.17504/protocols.io.2m3gc8n

Authors: Juan Carlos Herguera-García, Gisela Heckel

Summary: In the Stable Isotope Laboratory at CICESE (Ensenada Center for Scientific Research and Graduate Education, Mexico) animal tissues (pup fur from Pacific harbor seals, Phoca vitulina richardii) were ground for isotopic determinations. Samples were stored in a dissecator until they were loaded into a Costech® Carrousel. Samples were combusted at high temperature (1000˚C) in a pure oxygen atmosphere in an elemental analyzer coupled to a Finnigan MAT Delta V Advantage continuous flow stable isotope mass spectrometer.Stable carbon and nitrogen ratios are expressed as δ13C or δ15N according to the following equation:δ13C or δ15N (‰) = [Rsample/Rstandard)–1]* 1000 (1) where R is 13C/ 12C or 15N/ 14N. Isotopic values of carbon and nitrogen are reported relative to Pee Dee Belemnite and atmospheric nitrogen standards, respectively. The accuracy of isotopic measurements was verified using secondary standard reference materials (Glutamic acid, a pure carbonate Merck and the laboratory internal references Lanugo for N, and CH94 for C).

Associated Publications: Juárez-Rodríguez M, Heckel G, Herguera-García JC, Elorriaga-Verplancken FR, Herzka SZ, Schramm Y (2020) Trophic ecology of Mexican Pacific harbor seal colonies using carbon and nitrogen stable isotopes. PLoS ONE 15(1): e0225889. doi: 10.1371/journal.pone.0225889

Affiliations: Centro de Investigación Científica y de Educación Superior de Ensenada, Mexico (CICESE), Centro de Investigación Científica y de Educación Superior de Ensenada, Mexico (CICESE)

External URL: https://doi.org/10.1371/journal.pone.0225889

Version: 1

Publication Date: 2019

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