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URL: http://www.wormbase.org/db/get?name=WBStrain00054967
Proper Citation: RRID:WB-STRAIN:WBStrain00054967
Description: Caenorhabditis elegans with name fzy-1(lt20::loxP)/mIn1[mIs14 dpy-10(e128)] II. from WB.
Species: Caenorhabditis elegans
Synonyms: fzy-1(lt20::loxP)/mIn1[mIs14 dpy-10(e128)] II.
Notes: CRISPR/Cas9 engineered deletion of fzy-1 in which the fzy-1 coding sequence was replaced by LoxP. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are wild-type with relatively dim pharyngeal GFP signal, and segregate wild-type dim GFP (heterozygotes), Dpy bright GFP (mIn1 homozygotes), and non-GFP fzy-1 homozygotes (larval arrest). Pick wild-type with dim GFP and check for correct segregation of progeny to maintain. gRNA sequence: Ggacgcacgcccggtagtgc Reference: Kim T, et al. Genes Dev. 2017 Jun 1;31(11):1089-1094. doi: 10.1101/gad.302067.117. PMID: 28698300.
Affected Gene: WBGene00001072(dpy-10)|WBGene00001511(fzy-1)
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Source: Integrated Animals
Source Database: WormBase (WB)
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