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URL: http://www.wormbase.org/db/get?name=WBStrain00049003
Proper Citation: RRID:WB-STRAIN:WBStrain00049003
Description: Caenorhabditis elegans with name unc-5(lib1[myo-3p::GFP(-) + unc-119(+) + myo-2p::GFP(Mos1)]) IV; krIs14 V. from WB.
Species: Caenorhabditis elegans
Synonyms: unc-5(lib1[myo-3p::GFP(-) + unc-119(+) + myo-2p::GFP(Mos1)]) IV; krIs14 V.
Notes: Generated from afp_otherstrain Paper data WBPaper000612001.|"krIs14 [hsp-16.48p::MosTransposase + lin-15(+) + unc-122p::GFP] V. Recessive Unc. unc-5(lib1) is a CRISPR/Cas9 engineered mutant carrying the Intersister/Intrachromatid Repair Assay (ICR Assay) cassette inserted into the endogenous unc-5 locus. Briefly, ICR assay cassette includes two tandem GFP cassettes: the upstream using the myo-3 (body wall) promoter with a truncated GFP coding sequence, and the down-stream using the myo-2 (pharynx) promoter with GFP coding sequence interrupted by a Mos1 Drosophila transposon. Excision of Mos1 yields a single DSB, which if repaired by intersister or intrachromatid recombination, then will yield GFP+ progeny. The krIs14 insertion carrying heat-shock inducible Mos1 transposase is marked with coelomocyte GFP expression. Reference: Toraason E, et al. Current Biology 2021. https:"|"Made_by: Marissa Glover"
Affected Gene: WBGene00003514(myo-2)|WBGene00003515(myo-3)|WBGene00006745(unc-5)|WBGene00006843(unc-119)
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No rating or validation information has been found for unc-5(lib1[myo-3p::GFP(-) + unc-119(+) + myo-2p::GFP(Mos1)]) IV; krIs14 V..
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Source: Integrated Animals
Source Database: WormBase (WB)
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