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URL: http://www.wormbase.org/db/get?name=WBStrain00047292
Proper Citation: RRID:WB-STRAIN:WBStrain00047292
Description: Escherichia coli with name OP50 E. coli, rnc14::(delta)Tn10, lacz(gamma)A::T7pol camFRT from WB.
Species: Escherichia coli
Synonyms: OP50 E. coli, rnc14::(delta)Tn10, lacz(gamma)A::T7pol camFRT
Notes: Made_by: Jessica Sowa, Isaiah Neve|"Phage transduction (2x)"|"RNAi-capable OP50 strain. Tetracycline & Chloramphenicol resistant. Uracil auxotroph. E. coli B. Biosafety Level: BSL-1. Standard OP50 (CGC) was rendered RNAi competent through two modifications by phage transduction. The OP50 RNase III (rnc) allele was replaced with the deletion allele (rnc14::Tn10) found in HT115(DE3), and a genomically encoded IPTG-inducible T7 RNA polymerase (laczA::T7pol camFRT) was introduced. Reference: Neve IAA, et al. G3 (Bethesda). 2019 Nov 11. pii: g3.400741.2019."
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No rating or validation information has been found for OP50 E. coli, rnc14::(delta)Tn10, lacz(gamma)A::T7pol camFRT.
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Source: Integrated Animals
Source Database: WormBase (WB)