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URL: http://www.wormbase.org/db/get?name=WBStrain00037194
Proper Citation: RRID:WB-STRAIN:WBStrain00037194
Description: Caenorhabditis elegans with name acs-4(ok2872) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). from WB.
Species: Caenorhabditis elegans
Synonyms: acs-4(ok2872) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Notes: F37C12.7. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2872 homozygotes (sterile adult). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CTGTTTCAGGCAAATTGGGT. External right primer: TTCCTGTGCTCAAGTCGTTG. Internal left primer: ATGTTTGGGAACTCGACAGC. Internal right primer: ATCCTTGAACAACAGGGCAG. Internal WT amplicon: 1170 bp. Deletion size: 335 bp. Deletion left flank: CTGAAGACCCTGATTTATTTCGCTCCTGTG. Deletion right flank: AATTTTTATTGGATTTAAAACTCATTTTAC. Insertion Sequence: CGAAATA.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Affected Gene: WBGene00000254(bli-4)|WBGene00018152(acs-4)
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Source: Integrated Animals
Source Database: WormBase (WB)
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