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URL: http://www.wormbase.org/db/get?name=WBStrain00005062
Proper Citation: RRID:WB-STRAIN:WBStrain00005062
Description: Caenorhabditis elegans with name smg-1(cc546) I; rol-6(su1006) II. from WB.
Species: Caenorhabditis elegans
Synonyms: smg-1(cc546) I; rol-6(su1006) II.
Notes: Rollers. Maintain under normal conditions. Standard control for CL4176; originally used CL1175 as the control, but subsequently it was found that CL1175 can produce some A-Beta. Reference: Fonte V., et al. Mol Neurodegener. 2011 Aug 23;6(1):61. [NOTE: The temperature-sensitive allele cc546 causes an M1957L change in SMG-1. The lesion is an atg>ttg transversion in exon 35. Flanking sequences follow with the mutation site indicated with a capital A: ttggtggtcggttacaaaacgatattcaaga tcactggcagtcatgagtAtggttggatcagttttaggactcggtgatcg acatttggacaatttattg The lesion is detectable via SNP-snip with the mutation causing loss of an MslI site. Primers are for a 323 bp product. Digest with MslI to 86+237 in the wild type, uncut as 323 in the mutant. DJR701(f): CAGTCGTGAGCTTTGGATGCGTGC DJR702(r): TCGGGGATACGCAGATTCTTTCCC. Pedone ... Reiner G3 (2021).]|"Supplementary_genotype [smg-1(cc546) I; rol-6(su1006) II]"|"WBStrain provided so WBPaper00059477 paper added based on AFP_Strain data."
Affected Gene: WBGene00004397(rol-6)|WBGene00004879(smg-1)
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Source: Integrated Animals
Source Database: WormBase (WB)
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